FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498335
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13700.037.3116538
GTCTCGC15900.035.7796441
TCGCTCT16550.033.0638434
GTCTTGC31750.032.207031
CTCGCTC18550.027.946433
TCTCGCT18100.027.845642
CGCTCTG19750.025.882015
CTGTCGC21200.025.809799
GTATAAG9050.025.4628751
GTGTTAG22700.025.3787481
GTGTAAG16850.024.787581
GTGTAGA19100.023.7527141
GTATAAT9650.023.1334511
GTCTCAC37600.022.7910941
GTGTAAA19900.022.435961
GTACAGG18300.022.0365181
TTATACT9200.021.913314
GTCTTAC11200.021.8608461
TATACTG11300.021.662435
GTATTAT10400.021.4651741
Produced by FastQC (version 0.11.5)