FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498335
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG8700.032.276281
GTCTCGC13950.030.9681421
TCTGTCG14800.029.1958268
TCGCTCT15800.028.7075924
GTATTAG10000.027.3603531
GTATTAA11850.027.3421271
GTCTTGC31350.026.1821581
GTATAAT9450.024.3812681
GTATTAT12200.024.1970351
GTGTAAT14400.024.0003091
TTATACT10500.023.3132534
GTATAGG8750.023.0402971
TAATACT12200.023.0153754
CTCGCTC19100.022.61683
GTGTTAC14450.022.4224341
GTGTAGT21150.022.1279451
CTGTCGC20850.022.1057439
GTACTAT6250.021.8882831
TCTCGCT20200.021.7419222
GGAGTGC78000.021.6913475
Produced by FastQC (version 0.11.5)