FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384005
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14100.029.6217678
GTCTTGC28650.028.9038721
GTCTTAC13300.027.612221
GTCTCGC14900.027.0635361
GTATAAC5900.026.8505671
GTGTTAC11150.026.4784031
GTGTAAG16250.026.144571
GGTATAC7900.025.5219461
TCGCTCT15250.025.0221544
GTATAGA11150.024.5409581
GTCCGGA4053.45608E-1123.1137891
GTATAAG8900.022.6543081
CTGTCGC18000.022.0035259
GTATAAT7950.021.7383691
GTGTAAA18900.021.71681
GTCTCAC36150.021.114481
GAGTGCA78450.021.1083136
GGATAAT14250.020.718191
GTGTAAT12200.020.6581331
GTGTTAG18550.020.5738121
Produced by FastQC (version 0.11.5)