FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237487
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13200.047.451928
GTCTCGC13150.041.0782241
TCGCTCT14150.034.0947234
CTGTCGC19900.033.6463789
TCTCGCT15200.032.2137152
GTCTTGC27200.032.040011
CTCGCTC16700.030.6138113
GTATATA10200.027.5385231
GTATTAG11000.027.5000041
GTGTAAC8200.026.350181
GTCTTAC11650.025.347441
GTCTCAC31450.024.504131
GTGTAAG13600.024.3611
GTGTTAC10350.024.355881
CGCTCTG17600.024.1350275
TTGCTCT34500.023.5845834
GTATAAA9600.023.2576921
GTATAGA8250.022.6984181
GTATCGA2003.255018E-421.5988066
GTGTATA12500.021.3190521
Produced by FastQC (version 0.11.5)