FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2070962
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18950.036.4602241
TCTGTCG9600.035.994218
GTCTCGC9550.034.634581
TCGCTCT10650.030.4205824
GTATTAG6300.030.334271
GTGTAAC6250.029.4009081
GTATTAT6100.028.9189261
CTGTCGC12850.028.0110599
GTTGCGC1856.1436476E-627.2421533
GTACTAA4650.026.8717961
TTCACCG7400.026.2685815
TTACACT9700.025.9777134
GTATTAC4250.025.9419771
TCTCGCT12350.025.6724912
CGCTCTG11800.025.6254715
GTGTTAG13550.025.4952531
GTACTAT4101.8189894E-1225.0983331
GGTTAAC3551.3096724E-1024.8458371
CTCGCTC12350.024.4848483
GTCTCAC24400.024.0991041
Produced by FastQC (version 0.11.5)