FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164641
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8100.039.9965178
GTCTCGC8100.039.419111
TCGCTCT11100.033.0896644
CTGTCGC10900.031.7036639
GTCTTGC21350.031.609981
GTCTAAC5050.030.1763741
CTCGCTC11900.029.0542533
GTATAAT5850.028.5306131
GTCTCAC23600.027.6738531
CGCTCTG12250.027.6220845
TCTCGCT12100.026.7924732
GTATTAT6300.026.4927141
TTATACT5750.025.0499764
GTATAAC4201.8189894E-1224.1889971
GTGTTAG14850.023.9446661
GTTTCAC32550.023.6781753
GTGTTAA9400.023.159681
GTATTAA6650.022.9158941
GTGTAAC6650.022.9158941
GGGTTTC37600.022.7736851
Produced by FastQC (version 0.11.5)