FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164641
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9000.040.0348551
TCTGTCG10650.036.5016338
GTCTTGC20350.034.688981
TCGCTCT9500.034.100214
CTCGCTC11150.031.6372973
ACGCGTC1204.2312255E-429.9955527
TCTCGCT11800.029.9277482
TAATCCG1255.373555E-428.795735
CCGTTCG1753.991703E-628.7950679
CTGTCGC14250.027.7847149
CGCTCTG11850.027.3377195
GTGTTAC8900.027.2651981
GTACAAG8050.025.576841
GTGTTAG13000.025.4538731
ACCGTGT7100.025.3483548
GTATTAA7050.025.032651
GTATTAG6500.024.8882311
TTCACCG6850.024.1715985
CTTGCTC27800.024.0833173
GTCTCAC25250.023.8801571
Produced by FastQC (version 0.11.5)