FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2375833
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8300.042.5068471
TAGTACT5500.032.7247664
TCTGTCG10800.032.66218
GTCTTGC21800.030.681791
TCGCTCT10950.030.2442594
TCTCGCT11400.029.7094522
ATACCGA1555.77227E-527.8688346
GTCTCAC26800.026.8773881
GCGTTAT1657.672756E-526.7277931
CTCGCTC12650.026.1814673
CTGTCGC14600.026.1333359
GTACTAG5150.025.689821
TACCGTC1500.001557038523.9981617
GTGTTAC9800.023.2504521
CGCTCTG13150.022.9944365
TTGCTCT29650.022.8245584
ATAATAC7900.022.7845043
GGCTAGA13250.022.743841
GTTTCAC33900.022.7253573
GTATAAC4555.456968E-1222.6158241
Produced by FastQC (version 0.11.5)