FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141445
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8600.049.3914878
GTTCGTA604.943875E-448.3382341
GTCTCGC9350.046.528781
TCGCTCT10700.037.026344
TCTCGCT11900.035.7238852
CTGTCGC11900.035.0896959
GTCTTGC20800.034.1621171
CTCGCTC12300.030.4544223
TAGACCG950.0048931430.313425
GTGTAAA12150.027.4513421
GTCTTAC8550.027.1372551
GTCTCAC24900.027.0810571
GTATAGC4300.026.9794791
CGCTCTG13400.026.8635715
TACCCCG1907.5604275E-626.5242425
GTATAAT6100.026.150191
GTGTAAT8100.025.9594211
TGTTAGC13700.025.7708742
GTTAGCC12450.025.4586563
GTTTCAC30900.025.1777783
Produced by FastQC (version 0.11.5)