FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219088
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10050.045.1299978
GTCTCGC9650.042.1018641
CTGTCGC11100.041.5095339
GTCTTGC21200.038.3285831
TCGCTCT10300.032.868994
GTATTAC4450.030.9766311
GTATTAG6800.030.9406661
TCTCGCT12400.030.7948342
GGGTTTC34750.027.7675421
CTCGCTC13000.027.7058833
CGATACT1050.0079579527.440784
CCGTGTT7100.027.3776559
GTTTCAC33600.026.7988453
TTGCTCT28350.026.6785374
GTCTTAC9300.025.7437381
TTCACCG7300.025.6413755
GCGTAGT1701.0269703E-425.606071
CGCTCTG13300.025.441175
GTATTAA6100.024.9764141
GTGTTAC9800.024.430281
Produced by FastQC (version 0.11.5)