FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219088
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9850.043.1195538
GTCTCGC10300.040.7272151
GTCTTGC19750.035.772661
TCGCTCT11200.034.712284
CGCTCTG12200.032.457145
GTATTAG7600.030.0189271
CTGTCGC14650.029.976449
GTACGGT1753.3611705E-629.4379181
GTGTTAG14550.027.8193381
TCTCGCT13500.026.6885852
GTCTTAC9250.026.255441
GTATTAA5900.026.1947561
GTCTCAC24650.025.9746321
CTCGCTC13800.025.0437263
GTATAAT6500.024.9090061
TCTGTCA27050.024.749948
GTACCGT1500.001369873324.5315951
GTCTAAC4850.024.2786941
GGCTACG2751.7052116E-724.0855671
GTAACCG1500.001556223324.0002373
Produced by FastQC (version 0.11.5)