FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2145426
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10400.041.637321
TCTGTCG10150.036.8825078
GTCCGAT800.001918106336.69731
GTCTTGC17400.033.7446441
TCGCTCT11850.032.8071634
GTCTTAC8850.032.3433841
TCTCGCT12650.031.3270762
CTCGCTC12550.030.9780043
GTGTTAC9000.030.1733361
CTGTCGC14050.029.2059659
CGCTCTG12450.028.913055
GTATAAT5900.027.367481
GTATTAG6650.026.4882771
GTGTAAA11450.025.6400361
GTCTCAC24150.024.9207341
GCCCGTA1450.00127790124.8259247
GTTTCAC31350.024.8021663
TTGCTCT25800.024.5559234
GGGTTTC31800.024.4648691
GTATTAA6300.024.4648671
Produced by FastQC (version 0.11.5)