FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349120

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349120
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2096490
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10300.041.9374968
GTCTCGC8450.038.263121
GTCTTGC20050.036.283171
CTGTCGC13850.033.2657979
TAATACT7050.031.657124
TCGCTCT9800.029.3854774
CCGTGTT6400.025.8711419
TTGCTCT29050.025.2785914
CGCTCTG11150.025.1819045
TCTCGCT12150.024.9089452
ATACCGT1450.001277969824.8256636
CTCGCTC11100.024.6473273
GTTCGGC1500.001382079624.4941941
TATACTG6500.024.367345
GTGTAAT6950.024.3179761
GTTACCC6000.023.998713
TAGGTCG1500.001556986523.998145
GTGTTAG13500.023.9498791
GTCCTAC6950.023.2606741
GGATCGT3102.6082489E-823.2234528
Produced by FastQC (version 0.11.5)