FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054714
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.051.601988
CTGTCGC9750.045.7802129
TCGCTCT9500.041.7004974
GTCTCGC9050.041.664271
GTCTTGC19900.038.260021
CGTTTCG850.002838635833.8790979
TCTCGCT11250.032.017962
TTATACT6750.032.0125054
GTCGTAC1000.00607106829.0047421
CTCGCTC12450.028.9290733
CTTGTCG1753.9892384E-628.7972329
GTATAGA6550.028.783331
CGCTCTG12800.028.1222955
TTGCTCT26000.027.7031294
GTATTAT6450.026.9811531
TATACTG5350.026.9133015
GTATAAC5150.026.7519451
GTGTACG3005.857146E-1026.5876791
GTGTAAC6900.026.272411
GTCTTAC7750.026.197831
Produced by FastQC (version 0.11.5)