FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349124

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349124
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054714
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9700.047.7311971
TCGCTCT9750.045.7773174
TCTGTCG9300.037.929448
CGCTCTG11850.037.0573845
GTCTTGC17850.036.6425171
TCTCGCT12450.036.4660872
CTCGCTC11700.036.3036733
CGTTTCG1051.9328872E-434.2810869
GTACTAT5450.031.0145051
GTATTAA6950.030.6652661
TTGCTCT25700.028.2912124
GTGTACG2603.2214302E-928.2657281
CTGTCGC13600.028.0550359
GTATTAT7550.027.2549021
GTGTTAC10100.026.9224051
TATGCGC1100.00996256526.2050672
GTGTTAG13000.026.004471
GTCTCAC24650.025.3416881
CTTGCTC25100.024.9534133
ACTGTCG2351.4162597E-624.5067318
Produced by FastQC (version 0.11.5)