FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740724
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8250.050.6608661
TCGCTCT8600.045.208444
TCTGTCG7650.040.469818
TCTCGCT10750.035.5235252
CTCGCTC11000.033.4061283
CTGTCGC10350.033.3906639
GTCTTGC16800.031.3120981
CGCTCTG10400.031.153255
TAGTCCG1255.36907E-428.7994485
TAACACT8600.026.7901844
GTCTCAC20700.026.1089551
TACGACT1400.001041067225.7137954
GTATTAC4500.025.6215881
GTGTAAC5200.024.9440961
GTGTAGC9150.024.4140111
GTGTTAG13150.024.111571
GTATAGG4800.024.020241
GCGTAGC1801.584923E-424.020241
GTGTAAG9000.023.2195641
GTTAGCC9950.023.172643
Produced by FastQC (version 0.11.5)