FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740724
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7400.036.006061
GTATAGG4100.033.371471
CGCGAGA900.00375411631.9998742
GGTATAC3650.031.5669571
GTCTTGC16500.031.423471
GTATATA6250.031.1092381
TCGCTCT9150.029.9023784
GTGTTAC7400.029.1941051
TCTGTCG7600.028.4217558
CGCTCTG10150.028.3750865
GTACTAG3204.5474735E-1127.0045471
TAATACT4800.027.0006684
CTACGTC1358.417394E-426.6673283
GTATTAA4800.025.5042951
TCTCGCT10700.024.2242012
CTCGCTC11000.024.218783
GTATTAG5650.024.2164651
GTGTAAC5650.024.2164651
GTCTCAC21150.023.4933191
TTGCTCT22500.023.3605774
Produced by FastQC (version 0.11.5)