FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735060
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.041.65958
GTCTCGC8150.040.6723441
TCGCTCT8500.037.2698064
TCTCGCT9000.033.6196372
CGCTCTG8650.033.2940985
CTCGCTC9750.031.0335143
GTACCGT1402.8642884E-530.8831961
GTCTTGC17750.030.042241
CTGTCGC11350.029.1800049
GTCTTAC8100.028.468461
GTGTTAG12450.027.203671
GTATTAG5950.026.6443251
CTCGATC5850.025.8456128
TCGATCT6150.025.7555589
GTATTAA5750.025.064621
TTATACT6100.024.7863644
GTGTAAG9000.023.2195851
GTGTAAA10950.023.0331271
GTATATA8250.022.7100681
TCTCGAT6350.022.676697
Produced by FastQC (version 0.11.5)