FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856629
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7300.046.3553248
GTCTCGC8100.039.1483541
TCGCTCT8800.037.6356774
GGTATAC3200.036.0342831
CTGTCGC11200.033.4279639
GTCTTGC17250.032.1697351
TCTCGCT9200.032.1097222
CTCGCTC10600.030.5877253
GTCCGGA1902.1898086E-730.3446581
CGCTCTG10100.029.940055
TCGTACA1000.006285250728.7994774
GTGTAGG11400.026.5515751
GTATTAT5600.025.7387711
GTATAGA5600.025.7387711
GACGTAT1400.001041087725.7138188
GTGTTAG13100.025.306521
GTCTTAC6550.025.306521
ACCGTGT7200.024.9995488
GTACCGG2051.35215105E-524.6087781
CTGCGCG2651.4732359E-724.4523879
Produced by FastQC (version 0.11.5)