FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856629
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7950.043.4763531
GTATAGG4650.040.2623671
TCTGTCG7300.034.5205238
TCGCTCT9350.032.3422244
GTCTTGC17250.030.4728221
TCTCGCT10300.030.0582312
TATAGGG3900.029.538442
GTATTAG6800.027.5323561
CTCGCTC10050.026.5074443
GTACTAT4450.025.8904111
GTATTCG1400.001040327625.717041
TAATACT5650.025.4867084
CGCTCTG9650.025.3678575
GTCTTAC6650.024.904921
TACACTG12200.024.1967035
GTGTAAT5700.024.0025671
CTGTCGC12000.023.999989
GGAGTGC43900.023.7813035
GTTTCAC26250.023.5885543
GAGTGCA47400.023.5442876
Produced by FastQC (version 0.11.5)