FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067828
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8600.039.3480648
GTCTCGC8800.038.4900861
TCTCGCT10950.030.2635422
TAACGCA1204.2279728E-429.9994134
CGTTTCG1050.00797588927.4280329
CTGTCGC11800.026.8469319
TCGCTCT10700.025.5695924
GTCTTGC19450.025.5660231
CGCTCTG11000.025.526775
CTCGCTC11050.024.1221223
GTACTAG3902.1827873E-1124.0221821
GTATAAT5900.023.2078721
GGAGTGC48800.023.0159425
GTCTAAT6600.022.9302651
GTCTAGC9850.022.6808411
GTGCATA9600.022.5207981
TACACTA4800.022.499565
GTGTTAC8650.022.494761
TACTGCG2252.7994292E-522.3995597
GGGTTTC32250.022.1227531
Produced by FastQC (version 0.11.5)