FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850023
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7800.054.461678
GTCTCGC8000.046.8735961
TCGCTCT8650.044.9456564
CTGTCGC11500.041.3218429
TCTCGCT9100.039.6044242
CTCGCTC9300.039.5277753
CGCTCTG9550.035.4327245
GTCTTGC16700.034.977071
TACGACT1153.2951336E-431.302734
GCGTTAG1306.691057E-427.7358571
GTATTAC4250.027.148511
TTAAGCG1358.4213493E-426.665294
GTTAGCC9750.026.6141743
TTACACT8350.025.8669284
GGTATAC3701.0913936E-1125.337081
GTATATA8850.024.4451581
ACCGTGT5900.024.4068418
GTACTAT3551.6552804E-1024.37631
GTATAGC4600.023.515181
TGTTAGC12600.023.454622
Produced by FastQC (version 0.11.5)