FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1850023
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.049.0929348
GTCTCGC8150.040.6452061
GTCTTGC17200.038.0997471
TCGCTCT9150.033.837464
TTGCTCT20700.032.6970024
TCTCGCT9750.032.4918942
CGCTCTG10000.031.6813095
CTGTCGC14300.031.2180739
GTCGTAC950.00488688130.3211381
CTCGCTC10100.029.941833
GTGTTAG11150.029.7092781
CCGTACT1504.6120524E-528.801194
GTATAGC4300.028.470141
GTACTAT4200.027.433411
GTATAGT5200.026.3123361
GTGTAAT6450.025.678951
GTCTTAC7350.025.473881
CTTGCTC23350.024.3607483
GTCTCAC21400.024.2285751
GTCTACG1500.001554617124.0042341
Produced by FastQC (version 0.11.5)