FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098325
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.048.7618
CGTTTCG1201.0035023E-535.9993329
GTCTCGC10350.035.517381
GTCTTGC19300.035.4794921
TCGCTCT11050.034.532474
CTGTCGC14450.031.3904239
GTGTTAC9200.030.5553931
TTGCTCT25800.029.85924
TCTCGCT12800.029.2738742
GTATTAG7800.028.6469381
CGCTCTG12400.027.8697875
CTCGCTC12900.026.8125633
GTACCGC1358.421274E-426.6655376
CTTGCTC25050.026.1770343
GGGTTTC33850.025.9783941
GTTCTAC7050.025.5600681
GTTTCAC30450.025.3211443
ACCGTGT7500.024.9595368
GTATTAA5800.024.8549631
TAGTACT5350.024.2232724
Produced by FastQC (version 0.11.5)