FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2098325
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8950.034.592878
GTATTAG6700.032.2409821
GTCTCGC8950.032.1809431
GTCTTGC18100.029.4384521
GTACTAT5000.028.8019471
GTACTAG4150.027.760911
TATACCG1050.0079743827.4291155
TCGCTCT9650.026.8606364
GTCTCAC23750.026.3765181
GGGCTAC7100.026.3679771
CGTTACA1400.001041046825.7141822
GTGTTAC9100.024.5291271
TCTCGCT10900.024.4402712
GGGTTTC33350.024.397451
GTATTGG8050.024.15071
GACCGAA1500.001556104324.0004777
TAACCCG2101.6415286E-524.0004774
GAGTGCA49000.023.363736
GTCTTAC8650.023.3079321
CTGTCGC14350.023.0805979
Produced by FastQC (version 0.11.5)