FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2186540
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC19550.037.200141
TCTGTCG9750.034.708318
GTCTCGC10450.032.3855741
TCGCTCT10100.028.515364
GTATAAT6700.026.8679941
GTCTTAC9850.024.854941
TTGCTCT28500.024.7583354
GTACTAG5000.024.4821151
GTATTAA7650.024.4727021
CTAGCGT1500.001556144224.0004273
CTTGCTC27100.023.9118653
GTCTCAC22900.023.8972631
CTGTCGC14800.023.838269
GGTATAC5150.023.7690411
TCTCGCT12750.023.7175252
TAATACT7300.023.6716544
GGGTAAC7450.023.1966361
GTATTAG7850.022.931921
GAGTGCA54700.022.9035156
CTCGCTC12300.022.8296763
Produced by FastQC (version 0.11.5)