FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723581
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7150.049.344168
CTGTCGC10000.037.441149
GGTATAC3600.034.0435031
GTCTCGC8050.034.0310631
TCGCTCT7900.032.8092234
GTCTTGC16050.031.891221
ATTAACG1000.00626318128.8201073
GTATTAA5950.026.655911
TCTCGCT9300.026.3409582
GTCTTAC6600.026.2153191
GTATTAG6300.025.1750281
GTATTAT6100.024.8186021
CTCGCTC9600.024.767283
GTCTCAC20200.024.6255281
TTGCTCT24300.024.5919134
TACACTG12200.024.1960545
GTACCGG1801.5802367E-424.0307081
GTATAAA8200.023.7376521
CGCTCTG9750.023.6301175
GTGTTAC7650.023.5595191
Produced by FastQC (version 0.11.5)