FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1817996
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT7650.033.8822334
TCTGTCG7250.033.7653928
GTCTCGC7300.032.5523031
GTCTTGC15650.026.6872791
CTGTCGC10750.024.7813039
CTCGCTC9050.024.6628953
GTAATAA5350.024.2275411
GTCTCAC17650.024.071211
GTATAGG3602.0190782E-1024.0032141
GGAGTGC38100.023.8109365
GTGTTAG12600.023.431711
GTGCTAC4950.023.2758431
GACCCGT1550.001883404823.2257236
GTCTAGT6850.023.1271841
CGCTCTG9050.023.0717415
GTCTAAA6600.022.9121591
GGTCTAC4403.6379788E-1222.9121571
GTACTAA3503.792593E-922.6316011
GAGTGCA41950.022.4838286
GTATTAT6100.022.4292341
Produced by FastQC (version 0.11.5)