FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646137
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6500.038.772471
TCTGTCG8500.036.424368
TACCCGT1551.5468959E-632.516875
GTCTTAC5900.030.5110231
TCGCTCT7700.028.9876774
GTCTTGC15350.027.676581
GTGTAAC6300.027.4308621
CGTTTCG1050.00797377527.4291959
CTGTCGC12600.026.8577549
GTACTAG3255.638867E-1126.5868361
GTCTCAC19350.026.0486891
GTGTTAG12200.025.9693811
TCTCGCT9500.025.7682272
TTATTCG1400.001040788725.7148724
CTCGCTC8750.025.5091513
GTATTAG7350.025.4715161
ACAACGT2401.6980539E-624.0005478
GGGTTTC25650.023.8616431
GGGTAAT6050.023.8036421
GTTTCAC22450.023.733283
Produced by FastQC (version 0.11.5)