FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3788637
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11950.027.5311668
GTCTCGC11850.025.7058411
GTCGCCC11800.025.50827811
CTGTCGC12900.024.9610469
TGTCGCC12750.024.43118310
TCGCCCA12900.024.147112
GTCTTGC27050.023.428381
TCGCTCT13400.021.4211394
GTATAGA6850.019.9344831
GTATAGG7050.019.8656061
CGCTCTG13400.019.8537375
TCTCGCT14550.019.7280582
CTCGCTC15000.019.3695853
GTATTGG7550.019.0137521
GTACTAG4350.018.512691
GTGTACG3450.018.267721
TATACTG7250.017.864765
GTATTAG7950.017.616671
GTATATA8700.017.305341
GTCTTAC10600.016.8459421
Produced by FastQC (version 0.11.5)