FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3481323
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9300.024.4616328
TCGCTCT11900.022.6493074
GTCTCGC11750.022.3493861
GTATAGA5700.022.114131
CTGTCGC11450.021.0910729
GTCTTGC19900.020.9380421
GTATTAA5100.020.5964931
GTATTAT5600.020.008021
GTGTATA10350.019.9596941
TGTCGCC11150.019.77519210
TATACGG900.00527506719.4469342
TAATACT7650.019.2175944
GGTATAC4400.019.0985681
GTATTAG7400.018.9265061
GTACGGA1301.05516956E-418.8537121
GTACTAG4500.018.6741561
GTATAGG6000.018.6741521
CTCGCTC14300.018.3584673
GTGTAGA12450.018.280421
GTATACG1150.001005865618.2681921
Produced by FastQC (version 0.11.5)