FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195154
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT8250.024.1909051
GTCTCGC12800.022.977381
TCTGTCG10300.022.4273248
GTCTTGC25950.022.3976231
TCGCTCT12300.021.6279544
CTGTCGC12150.020.1647349
CGATCTA900.00528005419.44386937
TGTCGCC12300.018.78060310
GTATTAC6400.018.6007371
GTATATA10150.018.2827281
TCTCGCT14400.018.231672
CGATACT1351.4126032E-418.1497734
GTATAAG7050.017.8790571
GTCGCCC11000.017.8182911
GTCTTAC10750.017.5880011
GTGTAAA16200.017.2904451
CGCTCTG14550.017.0805665
GTATTAG9850.017.0622461
CTCGCTC14650.016.7252463
GTATTAA9200.015.9842651
Produced by FastQC (version 0.11.5)