FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4195154
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG501.7114673E-434.9992610
TCTGTCG10950.026.2101178
TGTCGCC12900.023.06152710
CTGTCGC13400.022.7239449
GTCTCGC12100.021.9955921
GTCTTGC25850.021.946331
GTATTAG8800.021.4891151
TAATACT6400.019.6870844
GTCGCCC13750.019.59841711
GTACGGA2002.4314431E-819.2606121
TCGCCCA15400.018.63552512
GTATTAC6000.018.0933041
CGTGTTA4550.017.69193510
GGCGTAC1401.8593125E-417.5096471
ATACCGC1200.001341953917.499846
GTGTAGT14050.017.4473361
GTATTAA8650.017.4084361
TCTCGCT14900.017.382392
GTACTAT6050.017.3649431
GTATAGC6100.017.2226051
Produced by FastQC (version 0.11.5)