FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3409646
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.027.0451668
GTATAAG5300.024.4444561
GTCTTGC22300.023.552711
GTCTCGC8850.022.1564031
GTATAGG5000.021.709321
GTCGCCC10750.021.16256311
TGTCGCC11450.021.09147610
CTGTCGC11800.021.0590979
GTATTAG6550.020.8486441
TCGCTCT10300.020.3911234
TAATACT6400.019.1432274
TCGCCCA12100.019.09070612
TTGCTCT27000.019.0581474
GTGTTAC8700.018.9161661
GTATTAA5200.018.8542461
GTATAGA5250.018.6746831
GTCTTAC9300.017.6957681
GTATACG1000.00967023217.5075151
GTGTTAG15900.017.3974061
CTTGTCG2259.4163624E-817.1109289
Produced by FastQC (version 0.11.5)