FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686590
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11100.022.396631
TCTGTCG9550.022.3564558
GTATAGA6200.022.0252191
GTATATA9100.021.5473541
GTATTAT6750.020.7493041
GTCTTAC10500.020.67521
CTGTCGC11250.020.2226129
GTCTTGC24700.019.9880071
GTATAGG5800.019.9220161
TCGCTCT12650.019.6457164
GTGTTAC9700.019.1316071
TAATACT7100.018.7337744
TGTCGCC11250.018.66677510
TACGATA950.007210484218.422414
TCTCGCT13800.018.0097942
GTATTAG8250.017.8255391
GGTATAC3203.6379788E-1217.5072251
GTGTAAC6650.017.3755931
CTCGCTC13950.017.3131033
CGCTCTG12600.017.223495
Produced by FastQC (version 0.11.5)