FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4024009
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.027.2400238
CTGTCGC12800.024.609569
TGTCGCC13150.023.68809710
CGACGAA750.001834274923.33234670
GTACGGG1854.0199666E-1022.7144451
GTCGCCC12150.022.46846611
GTCTCGC11600.021.1316131
TCGCCCA13350.019.66233412
GTACCGA900.00526335719.4545021
GTATAAC4000.019.2599561
GTGTTAT9100.018.8559021
GCGTAGT2306.0535967E-918.2703131
GTGTATA11150.017.5875661
CGTGTTA4600.017.49991210
CGCCGTA1401.867389E-417.49969767
TCGCTCT12500.017.0794934
TCTCGCT13450.016.9152552
GTCTTGC26550.016.882551
ACCGTGT7550.016.2252868
GTATAAA7800.016.16221
Produced by FastQC (version 0.11.5)