FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349761

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349761
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482360
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.025.185298
GTCTCGC10450.023.7916181
TATACCG1052.0400641E-523.3364475
TGTCGCC10850.022.9033210
GTATTAG7050.022.848131
CTGTCGC11500.022.5218329
GTCGCCC9850.021.67521711
TCGCTCT11100.021.443994
GTCTTGC24250.021.2269461
GTATCGC1003.922779E-421.0103441
GTGTTAC10000.020.311
GTATAGG5650.019.8327731
GTATTAT7000.019.5096041
TCGCCCA11950.018.74457712
GTATTGA6000.018.675861
GCGCTAG1351.4081644E-418.1570871
ATACGTT1351.411887E-418.150836
GGTATAC4650.018.0734121
TCTCGCT12400.018.068742
CTCGCTC12400.018.0666663
Produced by FastQC (version 0.11.5)