FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503259
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9200.027.0106778
CGAACGA657.965597E-426.92134914
TGTCGCC10300.025.4848910
CTGTCGC10650.025.3049839
GTCTCGC9850.024.5277461
TCGCTCT11900.022.3521424
GTCGCCC10500.021.66527611
TCTCGCT11250.021.1569162
GTATAGA6250.020.7284341
TACACTG15200.020.0327535
CGCTCTG11050.019.9546095
GTCTTGC23100.019.553411
CTCGCTC12800.019.4128153
CGTTTCG1107.48166E-419.0907739
GTCTTAC10400.018.8538231
GCGTAGT2801.0913936E-1118.7576311
TTATACT7100.018.7317264
GGTATAC4900.018.5789851
GTATAAT7600.018.428551
TCGCCCA12350.018.41960712
Produced by FastQC (version 0.11.5)