FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4121139
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12600.026.407461
TCGCTCT14300.024.4758074
TCTGTCG12300.023.6175868
CTGTCGC13400.022.7235939
TGTCGCC13350.020.97351810
TCTCGCT15600.020.6422672
CGCTCTG15600.019.9676975
CTCGCTC16150.019.070043
GTCGCCC12900.018.44781111
GTATTAC6700.018.2964481
CGTTTCG1150.001008705618.260649
GTCTTGC29150.017.9028151
GTGTTAC14050.017.4499931
GTGTATA15750.017.1231541
GGGTTTC45450.017.107741
GTATAAA11750.016.692591
GTGTAAC8400.016.6783941
GTATTAG8650.016.601271
GTGTTAG19550.016.482181
GTCTTAC12400.016.3824881
Produced by FastQC (version 0.11.5)