FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3531164
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10600.030.3773788
CTGTCGC12400.026.5322749
TGTCGCC12700.025.07875610
GTCTCGC11250.024.58581
GTCGCCC11050.024.38915411
TCGCTCT10950.022.056684
TCGCCCA12600.021.11112212
GTATAAT6800.020.5949551
GTGTTAC10450.019.7672141
GTATAAC4850.019.4908941
GTACTAT5500.019.0971411
GTCTTAC9650.018.8662591
TCTCGCT13550.018.8585032
CTCGCTC12450.018.8369523
GTCTTGC22450.018.7143461
GTATAGG5800.018.1093561
GTATTAA7400.017.9788381
CGCTCTG12800.017.7747065
GTATTAG8200.017.5057131
GTGTATA11000.017.5057131
Produced by FastQC (version 0.11.5)