FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375982
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10800.022.6858
TCGCTCT14250.022.5983724
GTCTCGC13800.021.820891
GTATTAT9100.020.3933051
CTGTCGC12550.020.35849
GTATAGA8150.019.763021
GTCTTGC27050.019.6756651
GTATTAC6850.018.9131661
GTATACG1501.49254865E-518.6746231
CTCGCTC15700.018.5049363
GGTATAC5300.018.4984471
TCTCGCT16500.018.0330412
TGTCGCC14200.017.99281110
CGCTCTG16400.016.861165
GTATAAA11850.016.8426191
TAGTACT6050.016.7782554
GTGTTAT10550.016.5947481
GTGTATA12400.016.3779451
GTCGCCC13750.016.29077711
ATAGTAC5850.016.1553753
Produced by FastQC (version 0.11.5)