FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265027
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCG450.004309603531.111349
CGAACGA703.4931436E-529.9998714
TCTGTCG11200.028.125218
GTCTCGC12450.023.3411671
CTGTCGC13450.023.160029
GTCTTGC27050.022.3919891
GTATTAG10100.022.1856651
TGTCGCC13100.022.17573510
TCGCTCT13200.022.0095424
CGTGTTA5200.020.19245710
GTATTAT9250.020.060791
GTCGCCC12450.019.39773411
CGTTTCG1451.1182607E-519.3104889
GTATTAC7500.019.1397571
TTCACCG8050.019.131925
GGGTTTC45800.018.1938781
CTCGCTC14900.017.8541563
GTTTCAC44900.017.5407493
GTACCGC1803.6514302E-617.5058751
GGTATAC6400.017.5058751
Produced by FastQC (version 0.11.5)