FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265027
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13400.026.379328
CTGTCGC14300.025.4533129
TGTCGCC15150.024.48726310
GTCTCGC13300.023.9585691
GTCGCCC13200.023.33165711
TCGCCCA14350.023.1696112
TCGCTCT14450.022.7667984
GTCTTGC29600.022.3584271
TCTATCG1454.6168316E-721.7230878
CGTGTTA5650.020.44148810
TCTCGCT15600.020.418792
GGTATAC6050.020.2574041
CGTTTCG1055.471683E-419.9990319
GTGTACG3250.019.393681
CGCTCTG15600.018.845025
GTATAAC4900.018.5801141
ACCGTGT9250.018.5396428
GTGTATA15050.018.1480181
GTACTAT6800.018.0231321
CTCGCTC16750.017.9686473
Produced by FastQC (version 0.11.5)