FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4496065
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11950.027.8399981
TCGCTCT13350.026.2162954
TCTGTCG11600.025.042498
CTGTCGC13350.024.119269
TGTCGCC12750.023.33224310
GTCGCCC11350.023.12564311
CGCTCTG15300.020.8162525
TCTCGCT15150.020.791342
TCGCCCA13200.020.41571212
GTATTAG10950.020.1483691
GTCTTGC28400.020.0993841
CGTTTCG1055.470863E-419.999519
CTCGCTC15750.019.7761943
GTATTAA9600.019.3338411
GTATTAT9650.019.2336651
TAATACT9650.018.8594344
ATCGTTC3800.018.42019310
GTATAGA8650.018.2183861
GTACTAG7300.017.7497541
GTGTTAC12850.017.7142891
Produced by FastQC (version 0.11.5)