FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349783

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349783
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080253
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.027.4170138
GTCTCGC12700.027.0184761
CTGTCGC13600.025.2209079
CGGTCGT851.3100344E-424.70528619
TGTCGCC13400.023.76895710
TCGCTCT13650.022.8227584
GTCGCCC12100.022.5622711
GTATCGC800.002662227721.8835891
TAATACT8500.020.590264
TCTCGCT14850.020.5085752
GTCTTGC28250.019.9547331
TCGCCCA14400.019.93080712
GTGTTAC9500.019.1654171
GTGTAAA16850.019.1172961
GTATAAT7250.018.8349781
CTCGCTC15600.018.6236293
GTATTAA7550.018.5503271
CGCTCTG15450.018.351325
GTGTAAT11950.018.1661261
GTGTAGA14500.017.8690831
Produced by FastQC (version 0.11.5)