FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647013
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14900.048.3281448
CTGTCGC17750.043.408219
GTCTCGC16750.041.508151
GTCTTGC35900.034.9002531
TCGCTCT17550.033.6424334
TCTCGCT18750.033.025382
CTCGCTC18450.031.607183
CGCTCTG20500.027.0450345
TAATACT12700.026.6467864
GTATTAT13150.026.4357991
GTCTTAC15050.025.504471
TTGCTCT51300.024.9835034
GTGTATA20950.024.198641
GTATTAG12050.024.0408541
GTATTAC9200.023.616221
GGTATAC9200.023.616221
CTTGCTC48300.023.1037083
GTATAAC7550.023.0219041
GTATTAA12900.023.0181851
GTATAAT11750.022.1891981
Produced by FastQC (version 0.11.5)