FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647013
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15250.042.0140768
GTCTCGC16500.035.1737061
CTGTCGC18950.033.8108029
TCGCTCT19800.030.1778034
GTCTTGC33300.028.2384431
GTATTAT11050.025.9284951
CGCTCTG22400.025.3894755
TCTCGCT22000.025.196992
CTCGCTC22450.025.012263
GTCTTAC16750.024.9997161
TTACACT20350.024.0557674
GTATAAT12250.023.3885611
ATGGCGT15400.023.3735126
GTATTAC9550.023.0777171
GGTATAC7800.022.604331
GTATAGG11600.021.5325721
GTATTAG13400.021.3813321
GTGTATA23400.021.3485321
ACCGTGT12650.021.0564848
GTATAAG11600.020.8992611
Produced by FastQC (version 0.11.5)