FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3904695
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16750.038.0486261
TCTGTCG14300.035.749688
CTGTCGC17000.033.036619
GTCTTGC32600.031.3236681
TCGCTCT20000.030.6004374
TCTCGCT20100.030.0930652
CTCGCTC21300.026.7022133
CGCTCTG20450.026.4062465
GTCTTAC14900.025.7608811
GTGTTAC15050.025.0229191
GTGTTAG24800.024.822091
GTGTATA17750.024.0726971
GTGTAAG18300.023.744951
GTTTCAC58600.023.342953
GGGTTTC63750.023.2886751
GTATTAC9350.023.2370381
GTGTAGA20450.023.019251
GTCTAGC15650.022.6752911
TTGCTCT47650.022.6655924
GTATAAG11200.022.6319071
Produced by FastQC (version 0.11.5)