FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3647292
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15350.045.023918
CTGTCGC19400.037.479529
GTCTCGC17800.035.9170341
TCGCTCT17550.035.687044
GTCTTGC34400.031.1885811
TCTCGCT22150.028.9257532
CGCTCTG20850.027.9670945
CTCGCTC22150.027.625723
CGTTTCG2105.31003E-727.4249029
GTGTTAC16050.026.5554811
GTACTAG7200.026.53641
GTCTCAC44250.024.7442441
GTGTTAG25700.023.446711
GTGTAGG20100.023.3983441
GTTTCAC55600.023.1764373
GGGTTTC61850.023.0495911
TTGCTCT48750.022.888934
GTGTAAG19300.022.4644551
GTATTAG12450.022.4292851
GTATTCG2953.406949E-722.419281
Produced by FastQC (version 0.11.5)