FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005349792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005349792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482789
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC15650.036.1557461
TCTGTCG13800.033.909468
GTATAAT9900.033.4024431
CTGTCGC16800.029.5683029
GTCTTGC33350.029.3060111
TCGCTCT16550.027.837564
TCTCGCT19100.026.7592642
CTCGCTC19700.026.6750873
GTGTTAG23050.025.1858771
GTATTAG11950.024.5976141
GTGTTAC13200.024.4951251
GTATAAG9350.024.3641361
GTGTTAT13600.024.3150141
GTGTAAG18150.024.2926861
GTATTAC7950.024.0329511
GTCTTAC14450.023.9018171
CTCGATC12650.023.3335028
CGTTTCG1550.00188486823.2233529
CGCTCTG19300.023.1250975
GTGTACG4850.022.7274341
Produced by FastQC (version 0.11.5)